NMDAR1、Caspase3在脊髓缺血性损伤的表达特点及相关性研究
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杨卫忠

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The expression and correlation of NMDAR1、Caspase-3 in spinal cord ischemia injury
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    目的探讨脊髓缺血后N-甲基-D-天门冬氨酸受体1(N-methyl-D-aspartatereceptor,NMDAR1)、Caspase-3的表达变化规律及意义。 方法 48只大白兔随机分为6组:假手术组(8只)、缺血6h组(8只)、缺血12h组(8只)、缺血24h组(8只)、缺血48h组(8只)、缺血72h组(8只)。手术组40只兔用结扎腰动脉方法建立脊髓缺血模型,假手术组(8只)只分离腰动脉而不结扎。分别运用反转录聚合酶链反应(RT-PCR)及蛋白印记技术在基因和蛋白水平检测NMDAR1、Caspase-3在不同缺血时间的表达变化规律,同时运用免疫组织化学技术观察NMDAR1、Caspase-3在细胞中的表达定位情况。结果 RT-PCR、蛋白印记显示缺血组与对照组比较NMDAR1、Caspase-3表达增加,并且有随缺血时间的延长表达逐渐增高的趋势;相关性分析显示NMDAR1、Caspase-3在 mRNA (r=0.947,p<0.005)、蛋白(r=0.984,p<0.005)水平呈正相关。免疫组化结果发现,NMDAR1、Caspase-3主要在细胞浆中表达。结论 脊髓缺血时NMDAR1、Caspase-3随缺血时间延长表达增加,NMDAR1、Caspase-3共同参与脊髓缺血性损伤过程,其表达与缺血时间有关。

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    Abstract Objective To study the diversity expression of NMDAR1、Caspase-3 in spinal cord ischemia injury. Method Fourty-eight rabbits were randomly devided into six groups as follows:sham-operated group and operated groups(n=8 at each time point) at designated times(6、12、24、48、72 hours after the surgery).The ischemia model was developed by selectively ligation of the five lubar arteries just inferior to the left renal vein and above the bifurcation. The mRNA and protein expression of NMDAR1、Caspase-3 in differerent times after the surgery were separately detected by reverse transcriptase-polymerase chain reaction( RT-PCR) and Western-blot ;at the same time ,where the NMDAR1、Caspase-3 located in the cell was detected by immunohischemistry. Results Compared with sham group,the level of NMDAR1、Caspase-3 in mRNA and protein was signifantly higher in the operated groups,there was significant difference between the two groups;meanwhile, NMDAR1、Caspase-3 expression in the spinal cord ischemia injury was time-related, the longer the ischemia time,the more expression of NMDAR1、Caspase-3 in spinal cord.; the correlation analysis indicated that there were positive correlation relation between NMDAR1 mRNA(r=0.947,p<0.005) and protein(r=0.984,p<0.005) in the same time. The NMDAR1、Caspase-3 protein was mainly located in the cytoplasm. Conclusion The expression of NMDAR1 、Caspase-3 during the spinal cord ischemia injury was time-related.This study indicated that NMDAR1、Caspase-3 were two important elements during the spinal cord ischemia.

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杨卫忠 贾建文 陈春美456. NMDAR1、Caspase3在脊髓缺血性损伤的表达特点及相关性研究[J].国际神经病学神经外科学杂志,2009,(5):0-111222. The expression and correlation of NMDAR1、Caspase-3 in spinal cord ischemia injury[J]. Journal of International Neurology and Neurosurgery,2009,(5):0-

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  • 收稿日期:2009-08-17
  • 最后修改日期:2009-09-21
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  • 在线发布日期: 2009-10-28
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