促红细胞生成素对脑缺血再灌注损伤保护作用的研究
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汤永红

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Study the protective effects of erythropoietin cerebral ischema-reperfusion injury
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    目的 探讨促红细胞生成素在脑缺血再灌注损伤中的保护作用机制。方法 采用Longa改良线栓法[1 ]制作局灶性脑缺血再灌注损伤模型,干预组于缺血前2 h、缺血前0 h 、缺血后6h予以促红细胞生成素3000 u/ kg[2]腹腔注射;缺血再灌注组、假手术组和正常组于相应的的时间给与等量生理盐水腹腔注射,并于缺血后2h拔出栓线。再灌注72 h 后进行神经功能缺损评分、Nissl小体、脑红蛋白和单核细胞趋化蛋白检测。结果:再灌注72 h后 , 缺血再灌注组Nissl小体明显少于干预组、假手术组和正常组;缺血再灌注组脑红蛋白、单核细胞趋化蛋白的表达量高于假手术组、正常组 ( P < 0. 01);同缺血再灌注组比较,干预组脑红蛋白明显增加( P < 0. 05),单核细胞趋化蛋白明显降低( P < 0. 01)。结论 促红细胞生成素可能通过上调脑红蛋白和下调单核细胞趋化蛋白表达而减轻脑缺血再灌注损伤。

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    [ABSTRACT] Aim To investigate protection of erythropoietin (EPO) on rat cerebral ischemia-reperfusion injury Methods The model of focal cerebral ischemia-reperfusion injury was made by occluding middle cerebral artery (MCA) . At 2hours or 0hour before or 6hours after cerebral ischemia , EPO intervention group received an intraperitoneal injection of rHu-EPO (3 000 u/kg), while ischemia-reperfusion group ,sham operation group and normal group received same doses of saline. The model was two hours after ischemia-reperfusion.The brains were removed 72hours after reperfusion.Neurological deficit scores apoptosis and expressions of Nissl body neuroglobin and monocyte chemoattractant protein-1 were detected. Results After 72 h reperfusion, Nissl body of ischemia-reperfusion group was significantly less than EPO-intervention group, sham-operated group, normal group. ischemia-reperfusion group neuroglobin and monocyte chemoattractant protein-1 expression was significantly higher than sham operation group and normal group (P <0. 01), with ischemia-reperfusion group compared with ,EPO-intervention group neuroglobin expression increased significantly (P <0. 05), monocyte chemoattractant protein-1 expression was significantly lower (P <0. 01). Conclusions EPO may be raised neuroglobin and lowered monocyte chemoattractant protein-1 expression, which can provide protective effect on cerebral ischemic-repefusion injury in rats.

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叶世武 汤永红 李赟456.促红细胞生成素对脑缺血再灌注损伤保护作用的研究[J].国际神经病学神经外科学杂志,2009,(5):391-394111222. Study the protective effects of erythropoietin cerebral ischema-reperfusion injury[J]. Journal of International Neurology and Neurosurgery,2009,(5):391-394

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  • 收稿日期:2009-07-11
  • 最后修改日期:2009-10-12
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  • 在线发布日期: 2009-10-28
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