Abstract: Objective To investigate the correlation between Glasgow Coma Scale (GCS) score and serum glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase (UCH-L1), interleukin-6 (IL-6) and early CT negative findings in patients with mild craniocerebral injury. Methods A total of 110 patients with mild craniocerebral injury admitted to the hospital from January 2019 to May 2023 were selected as research objects and divided into negative group and positive group according to CT findings. GCS score, GFAP, UCH-L1 and IL-6 were detected in the two groups. logistic regression analysis was used to screen the influencing factors of early negative CT findings in patients with mild craniocerebral injury, and Pearson correlation analysis was used to explore the correlation between GCS score, GFAP, UCH-L1 and IL-6 and early negative CT findings in patients with mild craniocerebral injury. Receiver operating characteristic curve (ROC) of GCS score, GFAP, UCH-L1 and IL-6 in the diagnosis of early negative CT findings in patients with mild craniocranial injury was plotted by Medcal software. Results GCS score was a risk factor for early negative CT appearance in patients with mild craniocerebral injury, and GFAP, UCH-L1 and IL-6 were protective factors for early negative CT appearance in patients with mild craniocerebral injury (P<0.05). GCS scores were positively correlated with early negative CT findings in patients with mild craniocerebral injury (r=0.612, P=0.000), and GFAP, UCH-L1 and IL-6 were negatively correlated with early negative CT findings in patients with mild craniocerebral injury (r=-0.606, P=0.000; r=-0.582, P=0.000; r=-0.670, P=0.000). The AUC of GCS score, GFAP, UCH-L1 and IL-6 for early negative CT findings in patients with mild craniocranial injury were 0.907, 0.867, 0.677 and 0.899, respectively. The AUC of the combination of GCS score, GFAP, UCH-L1 and IL-6 in the diagnosis of patients with mild craniocranial injury with early negative CT manifestations was 0.970. The AUC of GCS score, GFAP, UCH-L1 and IL-6 combined diagnosis of early CT negative features in patients with mild craniocranial injury was higher than that of GCS score, GFAP, UCH-L1 and IL-6 single indicators (P<0.05). Conclusion GCS score, GFAP, UCH-L1 and IL-6 can be used to diagnose early negative CT manifestations in patients with mild craniocerebral injury, and the combined detection of the four can improve the diagnostic accuracy.