微小RNA-21-5p调控PTEN、hMSH2、PDCD4促进胶质瘤表达
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1.宜昌市中心人民医院;2.襄阳市中心医院

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MiR-21-5p promotes glioma progression by regulating PTEN, hMSH2 and PDCD4
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1.The First College of Clinical Medical Science, China Three Gorges University&2.Yichang Central People’s Hospital;3.xiangyang Central Hospital

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    目的: 探讨微小RNA-21-5p(miR-21-5p)在胶质瘤中的生物学功能及其下游机制。方法: 下载miRNA表达谱数据集GSE41032,使用GEO2R在线分析工具筛选胶质瘤组织中差异表达的miRNA。采用实时定量PCR检测miR-21-5p在胶质瘤组织和细胞系的表达。细胞转染建立细胞模型。MTT、Transwell和流式细胞术检测细胞增殖活性、迁移、侵袭和凋亡能力。采用实时定量PCR和Western blot检测同源性磷酸酶-张力蛋白(phosphatase and tensin homolog,PTEN),MutS同源物2(mutS homolog 2,hMSH2)和程序性细胞死亡因子4(programmed cell death 4,PDCD4)的mRNA及蛋白表达水平。结果: miR-21-5p在胶质瘤组织和细胞中表达上调,下调miR-21-5p显著抑制U251细胞的增殖、迁移和侵袭,促进凋亡;过表达miR-21-5p则发挥相反的作用。PTEN、hMSH2和PDCD4被证明为miR-21-5p的下游靶点,并受到miR-21-5p的负向调节。结论: miR-21-5p通过靶向PTEN, hMSH2和 PDCD4在胶质瘤的进展中起到促癌作用。

    Abstract:

    Objective: To explore the biological function and downstream mechanism of microRNA-21-5p (miR-21-5p) in glioma. Methods: MiRNA microarray dataset GSE41032 was downloaded,and the differentially expressed miRNAs in glioma tissues were screened using GEO2R online analysis tool. Quantitative real-time PCR was used to detect the expression of miR-21-5p in glioma tissues and cell lines. Cell viability,migration,invasion and apoptosis were detected by MTT assay, Transwell assay and flow cytometry analysis, respectively. qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of photostase and tensin homologue (PTEN), human mutS homolog-2 (hMSH2) and programmed cell death 4 (PDCD4). Results: MiR-21-5p was up-regulated in glioma tissues and cells, and inhibition of miR-21-5p significantly suppressed the viability, migration and invasion of U251 cells, and promoted apoptosis; overexpression of miR-21-5p had the opposite effect. PTEN, hMSH2 and PDCD4 were identified to be downstream targets of miR-21-5p, and they were negatively regulated by miR-21-5p. Conclusion: MiR-21-5p can promotes the progression of glioma by targeting PTEN, hMSH2 and PDCD4.

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  • 收稿日期:2023-11-27
  • 最后修改日期:2024-08-17
  • 录用日期:2024-08-23
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