目的：探讨G蛋白偶联受体30（GPR30）在大鼠蛛网膜下腔出血 （SAH）早期脑损伤（EBI）过程中对神经炎症和血脑屏障（BBB）破坏的影响。 方法：36只雄性大鼠随机分为6组（n = 6/组）：假手术（Sham）组，SAH（3 h、6 h、12 h、24 h、72 h）组。 此外，72只大鼠随机分为4组（n = 18/组）：Sham组、SAH组、SAH联合过表达GPR30慢病毒阴性载体（SAH+Lv-NC）组、SAH联合过表达GPR30慢病毒载体（SAH+Lv-GPR30）组。通过血管内穿孔建立SAH模型，于SHA大鼠脑室内注射Lv-GPR30。通过神经学评分、脑组织含水量（BWC）检测、伊文思蓝（EBP）染色、苏木精和伊红（HE）染色来分析GPR30对EBI的影响；采用蛋白质印迹（Western Blot）和实时荧光定量PCR（qPCR）分析各种蛋白质和转录水平；通过酶联免疫吸附测定法（ELISA）分别测定肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、白细胞介素1β（IL-1β）、白细胞介素10（IL-10）水平。 结果：SAH大鼠脑内注射Lv-GPR30后增加了脑组织中GPR30的表达，并改善了大鼠神经功能，神经炎症，BBB破坏和脑水肿程度。过表达GPR30抑制SAH大鼠脑组织中基质金属蛋白肽酶9（MMP-9）和基质金属蛋白肽酶2（MMP-2）的表达，以及炎症因子TNF-α、IL-6、IL-1β水平，同时促进了IL-10的水平。 结论：GPR30减轻SAH大鼠的神经炎症和BBB破坏。
Objective: To investigate the effect of G protein-coupled receptor 30 (GPR30) on neuroinflammation and blood-brain barrier (BBB) during early brain injury (EBI) in rats with subarachnoid hemorrhage (SAH). Methods: 36 male rats were randomly divided into 6 groups (n = 6/group): sham operation (Sham) group, SAH (3 h, 6 h, 12 h, 24 h, 72 h) group. In addition, 72 rats were randomly divided into 4 groups (n = 18/group): Sham group, SAH group, SAH combined with overexpressed GPR30 lentivirus negative vector (SAH+Lv-NC) group, SAH combined with overexpressed GPR30 lentivirus Vector (SAH+Lv-GPR30) group. The Sprague-Dawley rat model of SAH was established by intravascular perforation. The sham group only punctured without puncturing blood vessels. Lv-NC and Lv-GPR30 were injected intraventricularly 7 days before modeling. The Changes of Neurological Function in rats were evaluated by using the improved Garcia score. The brain water content and Evans blue extravasation was performed 72 hours after the surgery to reflect the extent of cerebral edema and blood-brain barrier disruption. Hematoxylin and eosin (HE) staining to observe the pathological changes of brain tissue. Real-time fluorescent quantitative PCR (qPCR) analysis of the expression level of GPR30 mRNA in brain tissue. Expression levels of GPR30, matrix metalloprotein peptidase 9 (MMP-9) and matrix metalloprotein peptidase 2 (MMP-2) in rat brain tissue detected by Western Blot. The proinflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 1 beta (IL-1β), interleukin 10 ( IL-10) levels was detected by enzyme-linked immunosorbent assay(ELISA). Results: Compared with the Sham group, the neurological function of the rats in the SHA group was seriously impaired, the brain water content and Evans blue extravasation rate were significantly increased, the expressions of MMP-9 and MMP-2 in the brain tissue were increased, and the levels of TNF-α, L-6 and IL-1β in the brain tissue was significantly increased, and at the same time, the level of IL-10 in brain tissue was significantly inhibited. The difference is statistically significant (all P<0.05).As compared with SAH+Lv-NC group, the neurological function of the rats in the SHA+LV-GPR30 group was significantly improved, the brain water content and Evans blue extravasation rate were significantly reduced, the expression of MMP-9 and MMP-2 in the brain tissue was inhibited, and the The levels of TNF-α, IL-6 and IL-1β in tissues decreased significantly, while the levels of IL-10 in brain tissue increased significantly. The difference is statistically significant (all P<0.05). Conclusions: In summary, our results suggest that GPR30 can alleviate the function of the blood-brain barrier, thereby reducing cerebral edema, reducing neuroinflammation, and improving neurological function.