Abstract: Objective To investigate the expression and clinical significance of centromere protein F (CENPF) in brain glioma (Glioma). Methods By biological analysis of TCGA and CGGA databases, the expression differences of CENPF in paracancerous tissues and glioma tissues and the relationship between patients' prognosis were compared. The clinical data, glioma tissue specimens and 10 paracancerous tissue specimens of 60 patients undergoing brain glioma surgery in Linyi People's Hospital were collected. Using paracancerous tissue as control, immunohistochemical staining was used to detect the expression level of CENPF in 60 cases of glioma tissue and 10 cases of paracancerous tissue, and 12 cases of glioma tissue and 4 cases of paracancerous tissue were selected, and Western blotting was used to detect CENPF. Express. The survival data of all patients were followed up regularly, and the Kaplan-Meier survival curve was drawn according to the postoperative survival rate, and its relationship with clinicopathological parameters and patient prognosis was analyzed. Results Bioinformatics analysis showed that the expression of CENPF was positively correlated with the WHO grade of glioma, and the survival time of glioma patients with high CENPF expression was lower than that of patients with low CENPF expression. The results of immunohistochemistry and Western boltting experiments showed that the expression of CENPF was positively correlated with the WHO grade. The analysis of clinicopathological parameters showed that the expression of CENPF in glioma tissue was related to the expression of WHO grade, Ki-67 and P53 in patients (P<0.05), while the expression of CENPF in glioma tissue was related to the expression of WHO grade, Ki-67 and P53 (P<0.05). The age, gender, tumor location and MGMT of the patients were not related (P>0.05). Multivariate COX analysis showed that WHO grade, CENPF expression, P53 expression and Ki-67 expression (all P<0.01) were independent risk factors for poor prognosis of glioma patients. The Kaplan-Meier survival curve showed that the survival time of glioma patients with high CENPF expression was lower than that of patients with low CENPF expression, and the difference was statistically significant (P<0.0001). Conclusion The expression of CENPF is increased in glioma tissue, and its expression is correlated with WHO grade, Ki67 and P53 classification. CENPF can be used as a biomarker for judging the prognosis of glioma patients.