Abstract:Abstract Objective: To explore the effects of alanyl-glutamine combined with hyperbaric oxygen therapy on peripheral blood T-lymph subgroups, microRNA-124 (miR-124) and miR-181c in young and middle-aged stroke patients during the recovery period.Methods: 96 middle-aged and young patients with stroke recovery in our hospital from July 2017 to July 2021 were selected as the research objects, and they were divided into 3 groups according to the random number table method, with 32 cases in each group. The three groups were given conventional treatment during the recovery period of stroke. On this basis, the control group A was given alanyl-glutamine treatment, the control group B was given hyperbaric oxygen therapy, and the observation group was given alanyl-glutamine combined with hyperbaric oxygen The treatment was continued for 4 weeks. Compare the three groups before treatment, after 2 weeks of treatment, and 4 weeks after treatment. Nerve function (NIHSS score), ability of daily living (BI index), limb motor function (FMA score), inflammatory factors [tumor necrosis factor-α (TNF-α) , Monocyte chemoattractant protein-1 (MCP-1), interleukin-12 (IL-12)], peripheral blood T lymphoid subsets (CD4+, CD8+, CD4+/CD8+), cerebral hemodynamics [neck Arterial minimum blood flow velocity (Vmin), minimum blood flow (Qmin)], miR-124, miR-181c expression.Results: After 2 weeks and 4 weeks of treatment in the observation group, the NIHSS score was lower than that of the control group A and control group B, and the BI index was higher than that of the control group A and control group B (P<0.05); The observation group was treated for 2 weeks and 4 weeks later The motor function scores of upper and lower limbs were higher than those of control group A and control group B (P<0.05); Serum TNF-α, MCP-1 and IL-12 levels of observation group were lower than control group A after 2 weeks and 4 weeks of treatment Group, control group B (P<0.05); After 2 weeks and 4 weeks of treatment in the observation group, CD4+, CD4+/CD8+ were higher than control group A and control group B, and CD8+ was lower than control group A and control group B (P<0.05 ), and the CD4+, CD4+/CD8+ of control group A were higher than control group B, and CD8+ was lower than control group B (P<0.05); Vmin and Qmin of observation group were higher than those of control group A and control B after 2 weeks and 4 weeks of treatment Group (P<0.05), and the Vmin and Qmin of the control group B were higher than those of the control group A (P<0.05); The expression of miR-124 and miR-181c in the observation group was lower than that of the control group A and control after 2 weeks and 4 weeks Group B (P<0.05).Conclusion: Alanyl-glutamine combined with hyperbaric oxygen therapy can further improve the cellular immune function of the young and middle-aged stroke patients during the recovery period, down-regulate the expression levels of miR-124 and miR-181c, and significantly improve the patients' neurological function and ability of daily living.