Objective: To explore the value of modified aEEG score combined with S-100β and GFAP in the early diagnosis of epileptic encephalopathy in children.Methods: 92 children with status epilepticus who were treated in the department of pediatric neurology of our hospital from January 2018 to December 2020 were selected for the study.The clinical data of the children were collected, and aEEG examination was performed within 1 to 3 days after admission, and 2ml of fasting peripheral venous blood was drawn within 24 hours of admission to detect S-100β and GFAP.According to the presence or absence of epileptic encephalopathy, the children were divided into two groups, and the two groups were compared with the modified aEEG score combined with S-100β and GFAP.The ROC analysis method was used to evaluate the value of individual detection and combined detection of each index in the early diagnosis of epileptic encephalopathy in children with status epilepticus.Results: A total of 34 of the 92 children with status epilepticus in this study were confirmed to have epileptic encephalopathy. There was no significant difference in gender, imaging examination results and comorbidities between the two groups of children (P>0.05).The modified aEEG scores of children with epileptic encephalopathy were lower than those of the non-epileptic encephalopathy group, and S-100β and GFAP were higher than those of the non-epileptic encephalopathy group. The difference was statistically significant (P<0.05).ROC analysis results showed that the cut-off values of modified aEEG score, S-100β and GFAP for early diagnosis of epileptic encephalopathy in children with status epilepticus were 9 points, 1.16 μg/L, 4.61 ng/L, and AUC were 0.751 , 0.876 and 0.809,respectively, the AUC of the three combined diagnosis (referring to the improved aEEG score higher than the cut-off value or S-100β and GFAP lower than the cut-off value in the combined detection) is 0.908, and its diagnostic efficiency is higher than that of each indicator alone Detection (P<0.05).Conclusion: Children"s epileptic encephalopathy can cause modified aEEG score, S-100β, and GFAP changes. Modified aEEG score, S-100β, and GFAP changes can be used as early diagnostic indicators for childhood epileptic encephalopathy. The combined detection of the three can improve the diagnostic efficiency.