甘草酸二铵对颅脑损伤大鼠神经元活性、脑组织病理及YAP1蛋白的影响
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重庆市大足区人民医院,重庆 402360

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骆昌云(1986—),男,学士,Email: m13438842984_1@163.com。

通信作者:

代永龙(1987—),Email: fghre@163.com。

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Effect of diammonium glycyrrhizinate on neuronal activity, brain histopathology, and YAP1 protein in rats with traumatic brain injury
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The People’s Hospital of Dazu, Chongqing, Chongqing 402360, China

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    摘要:

    目的 探讨甘草酸二铵(DG)对颅脑损伤大鼠神经元活性、脑组织病理及YES关联蛋白1(YAP1)蛋白的研究。方法 将44只SD大鼠随机分为4组,每组11只,健康组、外伤组(颅脑外伤模型)、依达组(颅脑外伤模型给予依达拉奉干预)、甘草组(颅脑外伤模型给予DG干预)。采用Zea Longa法(5分)检测各组大鼠神经功能;用酶联免疫法测定血清白介素-6(IL-6)、IL-1β和肿瘤坏死因子-α(TNF-α)水平;采用HE染色观察脑组织神经细胞形态学变化;TUNEL法检测各组大鼠海马组织神经细胞凋亡;免疫印迹检测海马组织YAP1、磷酸化YAP1(p-YAP1)、大肿瘤抑制基因1(Lats1)及磷酸化Lats1(p-Lats1)蛋白表达水平。结果 与健康组相比,外伤组神经功能评分,IL-6、TNF-α、IL-1β,神经细胞凋亡、YAP1、p-YAP1、Lats1、p-Lats1均升高(P<0.05);与外伤组相比,依达组及甘草组以上指标均降低(P<0.05),依达组和甘草组比较,以上指标差异无统计学意义(P>0.05);与健康组相比,外伤组大鼠海马组织结构紊乱,神经元数目减少,细胞核深染,细胞膜部分消失,依达组和甘草组神经元排列比外伤组整齐有序,肿胀程度减轻,细胞核较清晰。结论 甘草酸二铵对颅脑损伤的治疗具有一定积极作用,且与依达拉奉治疗相关相当,其机制可能与改善神经功能、调节炎症因子变化及相关蛋白表达相关。

    Abstract:

    Objective To investigate the effect of diammonium glycyrrhizinate (DG) on neuronal activity, brain histopathology, and YAP1 protein in rats with traumatic brain injury.Methods A total of 44 rats were randomly divided into healthy group, trauma group (a rat model of traumatic brain injury), edaravone group (the rats with traumatic brain injury were given edaravone intervention), and DG group (the rats with traumatic brain injury were given DG intervention), with 11 rats in each group. The Zea Longa method (5 points) was used to evaluate the nerve function of the rats in each group; enzyme-linked immunosorbent assay was used to measure the serum levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α); HE staining was used to observe the morphological changes of neural cells in brain tissue; the TUNEL method was used to detect neuronal apoptosis in the hippocampal tissue of rats; Western blotting was used to measure the protein expression levels of YAP1, phosphorylated YAP1 (p-YAP1), large tumor suppressor gene 1 (Lats1), and extremely phosphorylated Lats1 (p-Lats1) in the hippocampus.Results Compared with the healthy group, the trauma group had significant increases in neurological score, IL-6, TNF-α, IL-1β, apoptosis of neural cells, YAP1, p-YAP1, Lats1, and p-Lats1 (P <0.05). Compared with the trauma group, the edaravone group and the DG group had significant reductions in the above indicators (P <0.05), while there were no significant differences between the edaravone group and the DG group (P >0.05). Compared with the healthy group, the trauma group had disordered structure of the hippocampus, with a reduction in the number of neurons, hyperchromatic nuclei, and partial disappearance of cell membrane, and compared with the trauma group, the edaravone group and the DG group had ordered arrangement of neurons, with alleviation of swelling and clear nuclei.Conclusions Diammonium glycyrrhizinate has a positive effect on the treatment of cranial injury, which is similar to that of edaravone. Its mechanism may be related to improving nerve function, regulating the changes of inflammatory factors and the expression of related proteins.

    表 4 各组大鼠YAP1、p-YAP1、Lats1、p-Lats1蛋白表达量Table 4
    表 1 Zea Longa评分Table 1
    图1 脑组织病理比较(HE,×400)Fig.1
    图2 海马组织神经细胞凋亡情况(TUNEL,×400)Fig.2
    图3 各组大鼠脑组织中YAP1、p-YAP1、Lats1、p-Lats1蛋白表达比较Fig.3
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骆昌云,代永龙,谭正芳456.甘草酸二铵对颅脑损伤大鼠神经元活性、脑组织病理及YAP1蛋白的影响[J].国际神经病学神经外科学杂志,2024,(2):18-22111LUO Changyun, DAI Yonglong, TAN Zhengfang222. Effect of diammonium glycyrrhizinate on neuronal activity, brain histopathology, and YAP1 protein in rats with traumatic brain injury[J]. Journal of International Neurology and Neurosurgery,2024,(2):18-22

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  • 收稿日期:2022-05-26
  • 最后修改日期:2024-01-31
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  • 在线发布日期: 2024-06-19
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