Duchenne muscular dystrophy （DMD） is a X-linked recessive genetic disease associated with a single gene. DMD primarily affects males and has the symptom of severe and progressive muscle weakness. This condition is highly debilitating and life-threatening and has no effective treatment. However， emerging clinical data indicate that female carriers of DMD can also demonstrate various degrees of clinical symptoms. Consequently， it is vital to screen for DMD carriers to decrease the incidence of children born with this disease. The guidelines can help to identify the etiology for early prevention and guide family members regarding further reproductive decisions， thereby advancing the standardized prevention and control of this disease.