Objective To investigate the effect of virtual reality （VR） technology combined with limb coordination assist device training on cerebral gray matter content， motor function， cognitive function， and activities of daily living in patients with ischemic stroke.Methods A total of 122 patients with ischemic stroke who were admitted to The Second People's Hospital of Hefei from February 2022 to January 2023 were enrolled as subjects， and they were divided into control group and observation group using a random number table， with 61 patients in each group. The patients in the control group were trained with the limb coordination assist device， and those in the observation group were trained with VR combined with the limb coordination auxiliary device， with a treatment time of 2 months for both groups. The two groups were compared in terms of 1 min eye opening track area， 1 min eye opening swing length， motor function of the upper and lower limbs， neurological function， cognitive function， and activities of daily living before and after treatment.Results After treatment， both groups had significant reductions in 1 min eye opening track area and 1 min eye opening swing length（P<0.05）， and the observation group had significantly smaller area and length than the control group（P<0.05）. After treatment， both groups had significant increases in the motor scores of the upper and lower limbs， grey matter content， Montreal Cognitive Assessment score， and activities of daily living（P<0.05）， and these indicators were significantly higher in the observation group than in the control group（P<0.05）. After treatment， both groups had a significant reduction in neurological score（P<0.05）， and the observation group had a significantly lower score than the control group （P<0.05）.Conclusions For patients with ischemic stroke， VR combined with limb coordination auxiliary device training can effectively promote the recovery of balance function and improve limb motor function， neurological function， cognitive function， and abilities of daily living. ［Journal of International Neurology and Neurosurgery, 2023, 50(6): 19-23］