血管结构蛋白、生成因子和基质蛋白酶在颅内动脉瘤破裂进程中的表达规律研究
作者:
作者单位:

1.新疆医科大学第一附属医院昌吉分院神经医学科,新疆 昌吉 831100;2.新疆医科大学第一附属医院神经外二科,新疆 乌鲁木齐 830054

作者简介:

通信作者:

杜郭佳,Email:dgrjav@163.com。

基金项目:

新疆维吾尔自治区自然科学基金面上项目(2019D01A87)。


Expression of vascular structural proteins, growth factors, and matrix proteases during the course of ruptured intracranial aneurysms
Author:
Affiliation:

1.Department of Neuroscience, Changji Branch, the First Affiliated Hospital of Xinjiang Medical University, Changji, Xinjiang 831100, China;2.Department of Neurosurgery II, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 音频文件
  • |
  • 视频文件
    摘要:

    目的 拟通过同一患者的不同阶段的颅内动脉瘤(IA)血管结构蛋白、生长因子和基质蛋白酶的表达进行分析,探索动脉瘤破裂的分子机制,为IA的早期筛查和预防提供理论依据。方法 采集6名IA患者未破裂动脉瘤、破裂动脉瘤的动脉瘤壁和颞浅动脉血管壁样本。通过qRT-PCR和Western blotting检测3种结构蛋白(III型胶原、纤维连接蛋白和层粘连蛋白)、2种血管生成因子(血管内皮生长因子、碱性成纤维细胞生长因子)和2种基质蛋白酶的表达水平变化趋势。结果 正常血管壁样本(颞浅动脉)中III型胶原、层粘连蛋白、成纤维细胞生长因子和基质金属蛋白酶2呈现高水平表达;未破裂动脉瘤样本中,大多数蛋白趋于中度表达水平;动脉瘤破裂后III型胶原和层粘连蛋白表达水平降低,纤维连接蛋白和血管内皮生长因子表达水平升高。mRNA水平表达与蛋白表达水平基本一致。结论 正常血管壁、未破裂动脉瘤壁和破裂动脉瘤壁中结构蛋白和血管生成因子表现出不同的表达水平和模式。 [国际神经病学神经外科学杂志, 2022, 49(3): 14-20.]

    Abstract:

    Objective To investigate the molecular mechanism of rupture of aneurysm by analyzing the expression of vascular structural proteins, growth factors, and matrix proteases in one patient with intracranial aneurysm (IA) at different stages, and to provide a theoretical basis for the early screening and prevention of IA.Methods The samples of the unruptured aneurysms, the ruptured aneurysm wall, and the superficial temporal artery wall were collected from 6 patients with IA. Quantitative real-time PCR and Western blotting were used to measure the changing trends of the expression levels of three structural proteins (type III collagen, fibronectin and laminin), two growth factors [vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)], and two matrix proteinases [JNK and matrix metalloproteinase 2 (MMP2)].Results There were high expression levels of type III collagen, laminin, bFGF, and MMP2 in the normal vascular wall samples collected from the superficial temporal artery. Most proteins showed a moderate expression level in the samples of unruptured aneurysms. After rupture of aneurysms, there were reductions in the expression levels of type III collagen and laminin and increases in the expression levels of fibronectin and VEGF. The mRNA expression levels were basically consistent with the protein expression levels.Conclusions Structural proteins and growth factors show different expression levels and patterns in normal vascular wall, unruptured aneurysm wall, and ruptured aneurysm wall. [Journal of International Neurology and Neurosurgery, 2022, 49(3): 14-20.]

    参考文献
    相似文献
    引证文献
引用本文

白旭升,冯志芳,赵常发,阿迪力江·艾则孜,杨文娟,杜郭佳.血管结构蛋白、生成因子和基质蛋白酶在颅内动脉瘤破裂进程中的表达规律研究[J].国际神经病学神经外科学杂志,2022,49(3):14-20

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2021-12-02
  • 最后修改日期:2022-05-26
  • 录用日期:
  • 在线发布日期: 2022-07-29
关闭
我刊在喜马拉雅FM开通账号并传播文献

关闭