The blood-brain barrier （BBB） is a dynamic and highly compact tissue structure that strictly regulates the exchange of substances between the brain and peripheral blood， and the destruction of BBB is associated with a variety of central nervous system diseases. The construction of a suitable BBB model is beneficial to the research on central nervous system diseases and drug screening. In this paper， we review the research progress of in vitro BBB model， and mainly discuss the construction methods and characteristics of the most widely used Tran swell model and the latest micro fluidic chip model. The most commonly used model is the Tran swell model， which can directly determine the transendothelial resistance and permeability of BBB. While， the micro fluidic chip model is a more promising in vitro model because the BBB micro fluidic chip simulates the blood flow through the micro perfusion system， which is more in line with the physiological state of organs and tissues， and is more suitable for the construction of disease model， drug screening， and so on. ［Citation:Journal of International Neurology and Neurosurgery, 2021, 48(3): 303-306］