长期丰富环境对由神经毒素MPTP所致的帕金森病小鼠模型抑郁情绪的作用
作者:
作者单位:

1.新乡医学院第一附属医院神经内科/河南省神经修复重点实验室,河南 新乡 453100;2.新乡医学院基础医学院生理学与神经生物学教研室,河南 新乡 453000;3.新乡医学院第三附属医院神经内科,河南 新乡 453003

作者简介:

车祥源(1993-),男,硕士,研究方向:帕金森病的基础与临床研究。

通信作者:

邢红霞(1972-),女,主任医师,教授,医学博士,硕士生导师,研究方向:帕金森病的基础与临床研究。Email:xhxwh02@163.com。

基金项目:


Effect of long-term environmental enrichment on depressed mood in a mouse Parkinson’s disease model induced by neurotoxin MPTP
Author:
Affiliation:

1.Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University/Henan Provincial Key Laboratory of Nerve Repair, Xinxiang, Henan 453100,China;2.Department of Physiology and Neurobiology, School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453000, China;3.Department of Neurology, Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, China

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    摘要:

    目的 探讨长期丰富环境(EE)对由神经毒素MPTP所致的帕金森病(PD)小鼠模型抑郁情绪的作用。方法 采用随机分组法将40只7月龄雄性C57BL/6N小鼠,分为EE+MPTP组、EE+NS组、SE+MPTP组与SE+NS组,每组10只。EE组和标准环境(SE)组在干预3个月后,MPTP组小鼠进行神经毒素MPTP造模(腹腔注射,30 mg/kg),每天注药1次,连续7 d;NS组给予腹腔注射等量的0.9%NaCl作为对照观察。采用行为学方法评估小鼠行为学表现。行免疫组织化学染色方法观察酪氨酸羟化酶(TH)与5-HT1A受体阳性面密度值变化情况。结果 行为学结果显示MPTP组的小鼠的强迫游泳评分较NS组小鼠低(P<0.01);悬尾实验静止不动时间延长(P<0.01)。EE+MPTP组的上述抑郁表现轻于SE+MPTP组(P<0.05)。从免疫组织化学染色结果中发现,SE+MPTP组和EE+MPTP组小鼠的TH与5-HT1A受体阳性表达水平较NS组小鼠降低(P<0.01),而EE+MPTP组小鼠TH及5-HT1A受体阳性表达水平降低程度较SE+MPTP组小鼠小(P<0.05)。结论 MPTP小鼠的行为学异常以及TH阳性面密度值减少提示PD模型制作成功。EE+MTPT组小鼠5-HT1A受体受损较SE+MPTP组小鼠轻,提示丰富环境可能缓解MPTP诱导的PD小鼠模型的抑郁情绪。国际神经病学神经外科学杂志,2021,48(3): 239-243]

    Abstract:

    Objective To investigate the effect of long-term environmental enrichment (EE) on depressed mood in a mouse Parkinson’s disease (PD) model induced by neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).Methods A total of 40 seven-month-old male C57BL/6N mice were randomly divided into EE+MPTP (n=10), EE+normal saline (NS)(n=10), standard environment (SE)+MPTP (n=10), and SE+NS (n=10) groups. After three months of intervention with EE or SE, the mice in the MPTP groups were subjected to neurotoxin MPTP (intraperitoneal injection, 30 mg/kg) once daily for 7 consecutive days for modeling. The mice in the NS groups were given intraperitoneal injection of the same amount of 0.9%NaCl as the control study. Behavioral methods were used to evaluate the behavioral manifestations of mice, and immunohistochemical staining was performed to observe the positive areal density values of tyrosine hydroxylase (TH) and 5-hydroxytryptamine 1A (5-HT1A) receptor.Results The behavioral results showed that the mice in the MPTP groups had a significantly lower forced swimming score (P<0.01) and a significantly long erimmobility time in the tail suspension test (P<0.01) than those in the NS groups. The mice in the EE+MPTP group had significantly milder depression symptoms mentioned above than those in the SE+MPTP group (P<0.05). The results of immunohistochemical staining showed that the mice in the SE+MPTP group and EE+MPTP group had significantly lower positive expression levels of TH and 5-HT1A receptor than those in the NS groups (P<0.01), while the mice in the EE+MPTP group had significantly smaller decreases in positive expression levels of TH and 5-HT1A receptor than those in the SE+MPTP group (P<0.05).Conclusions The behavioral abnormalities of MPTP mice and the reduction in TH positive areal density value suggested that the PD model was successfully established. The mice in the EE+MTPT group had significantly milder 5-HT1A receptor damage than those in the SE+MPTP group, suggesting that environmental enrichment may alleviate the depressed mood in the MPTP-induced PD model in mice. [Citation:Journal of International Neurology and Neurosurgery, 2021, 48(3): 239-243]

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车祥源,郑志勇,李超堃,邢红霞.长期丰富环境对由神经毒素MPTP所致的帕金森病小鼠模型抑郁情绪的作用[J].国际神经病学神经外科学杂志,2021,48(3):239-243

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  • 收稿日期:2020-12-29
  • 最后修改日期:2021-03-30
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  • 在线发布日期: 2021-08-27
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