Glioblastoma (GBM) is the most common malignant tumor of the central nervous system in adults, with high heterogeneity and complex interactions between tumor cells and the tumor microenvironment. Current in vitro models cannot accurately reproduce the brain tumor microenvironment, and in vivo in situ models are time-consuming, costly, and often not successful. Organoids as a new model system can accurately simulate the physiological structure and function of in situ tumor tissues, and can maintain the characteristics of tumor cells in vivo. Cerebral organoids have been widely used in the study of various malignant tumors. Gene-editing technologies have been established to induce brain tumors in cerebral organoids to study the early stages of tumorigenesis. Cocultivation of GBM stem cells and cerebral organoids can be used for high-throughput drug screening. Alternatively, GBM organoids can be directly established and cultured to study tumor biology and predict treatment response. In this article we briefly introduce the cerebral organoids, review the various experimental techniques for the establishment of GBM organoids, and summarize the applications of GBM organoids.