Objective To investigate the role of internal fixation in resection of spinal canal tumors.Methods A retrospective analysis was performed on the clinical data of 21 patients with spinal canal tumors who were treated by internal fixation combined with microsurgery from October 2016 to April 2019. The spinal canal tumors were removed by microsurgery under neurophysiological monitoring, and the spinal stability was maintained by internal fixation.Results Among the 21 tumor cases, 19 had a total resection, 2 had a near-total resection, 1 had a postoperative recurrence, and 1 had a metastasis to the third ventricle. There were no aggravation of neurological function injury after surgery, injury of nerves, spinal cord, and blood vessels during internal fixation, postoperative infection, or loosening, rupture, and disarticulation of internal fixation during follow-up. Ninety-five point two percent of the cases achieved an osseous fusion.Conclusions Internal fixation combined with microsurgery can resect spinal canal tumors and maintain spinal stability, which is helpful in expanding exposure area and protecting spinal nerve function.