Objective To investigate the expression and function of non-coding microRNA-195 (miR-195) in meningioma.Methods In situ hybridization was used to measure the expression of miR-195 in meningioma and its correlation with clinical classification was analyzed. After miR-195 was overexpressed in meningioma IOMM-Lee cell line, CCK8 and flow cytometry were used to investigate the influence of miR-195 on the biological function of meningioma cells.Results MiR-195 was expressed in meningioma, and its expression level was negatively correlated with tumor grade. Overexpression of miR-195 in meningioma cells significantly inhibited the viability of tumor cells and helped to achieve cell cycle arrest in G1 phase. The results of luciferase reporter gene assay showed that miR-195 directly inhibited the transcriptional level of c-myb expression, and overexpression of miR-195 in meningioma cells significantly inhibited the mRNA and protein expression of c-myb.Conclusions MiR-195 is abnormally expressed in meningioma, which is correlated with clinical tumor classification. MiR-195 inhibits the cell cycle of meningioma cells and the growth of tumor cells by targeted regulation of the expression of c-myb.