Objective To investigate the association between B-cell CLL/lymphoma 3(BCL3) gene expression and the prognosis of glioma.Methods The glioma datasets containing BCL3 gene expression and prognosis of patients were downloaded from Chinese Glioma Genome Atlas and The Cancer Genome Atlas.GeneSpring GX 11.0 software was used for normalization,and Cox regression analysis and Kaplan-Meier survival analysis were used for data analysis.Results In the datasets of low-grade glioma and glioblastoma,BCL3 gene expression was significantly correlated with overall survival (OS) and disease-free survival (P<0.05).In the datasets of Batch1 and Bactch2,BCL3 gene expression was significantly correlated with OS (P<0.05).Based on the median of BCL3 gene expression,each dataset was divided into high-BCL3 expression group and low-BCL3 expression group;the high-BCL3 expression groups had a significantly higher risk of death than the low-BCL3 expression groups,and in the glioblastoma multiforme dataset,the high-BCL3 expression group had a significantly higher risk of recurrence than the low-BCL3 expression group.Conclusions BCL3 gene expression is significantly correlated with the prognosis of glioma patients,and high BCL3 expression is associated with poor prognosis.BCL3 gene expression may be a new prognostic factor for glioma.