Objective To investigate the clinical effect of microsurgical resection of intraspinal tumors under multimodal neurophysiological monitoring.Methods A retrospective analysis was performed for the clinical data of 57 patients with intraspinal tumors who underwent microsurgical resection of intraspinal tumors under multimodal intraoperative monitoring of somatosensory evoked potential (SEP),motor evoked potential (MEP),and electromyography (EMG).Results Of all patients,49(86.0%) underwent total resection,6(10.5%) underwent subtotal resection,and 2(3.5%) underwent partial resection.Four patients were lost to follow-up,and the other 53 patients were followed up for 3 months to 2 years after surgery,among whom 45(78.9%) achieved improvement in neurological function,4(12.3%) had no significant improvement,3(7.0%) had aggravated symptoms,and 1(1.8%) experienced recurrence.Conclusions Microsurgical resection of intraspinal tumors under multimodal neurophysiological monitoring can effectively improve the total resection rate of tumors,predict and protect the function of spinal nerves,and improve surgical outcome and safety.