Objective To investigate the expression of H19 (a long noncoding RNA) in human glioblastoma multiforme (GBM), its effect on the biological function of U87-GBM, and the mechanism.Methods The expression of H19 in GBM was determined by the real-time quantitative PCR detection system. The Cell Counting Kit-8 was used to determine the proliferation of U87 cells. Flow cytometry was used to assess the apoptosis and cell cycle of U87 cells. The migration and invasion of U87 cells were detected by transwell assay. Western blot was used to measure the expression of p53.Results H19 was highly expressed in GBM. The overexpression of H19 promoted the proliferation, invasion, and migration of GBM and inhibited the apoptosis of GBM. In addition, the overexpression of H19 increased the proportion of cells in S phase and inhibited the expression of p53.Conclusions H19 can promote the carcinogenesis of GBM.