Objective To investigate the expression of Rab30 in PC12 cells and possible pro-apoptotic pathways under hydrogen peroxide (H₂O₂) intervention.Methods PC12 cells cultured in vitro were divided into normal group and H₂O₂ group. MTT assay was used to measure cell viability, Annexin V-FITC/PI double staining was used to measure apoptosis rate, and Western blot was used to measure the protein expression of Rab30, JNK3, GM130, and caspase-3.Results H₂O₂ intervention induced a significant reduction in the survival rate of PC12 cells (P<0.05) and significant increases in early apoptosis rate and overall apoptosis rate (P<0.05); the PC12 cells had significant increases in the protein expression of JNK3 and caspase-3 (P<0.05) and significant reductions in the protein expression of Rab30 and GM130 (P<0.05).Conclusions The pro-apoptotic pathway of H₂O₂ in PC12 cells may be activation of JNK3 expression, targeted down-regulation of Rab30, destruction of Golgi structure, and activation of caspase-3, which induces the apoptosis of PC12 cells.