Objective To explore the influence of different testing time to the result of encephalofluctuograph (EFG). To study the reliability of encephalofluctuograph.Methods 120 healthy people were included in this study. They were taken EFG testing totally four times within two days. During 9:00-10:00 am and 14:00-15:00 pm.Results There was not significant differences in EFG result between T₁-T₂, T₃-T₄, T₁-T₃, T₂-T₄ (P>0.05).Conclusions There is no effect of different testing time on the result of EFG is stable for clinical application with high reliability.