Objective To study the relationship between gene polymorphisms of blood coagulation factors (factor II, FII; factor V, FV) and cerebral infarction in the Han Chinese population from Changsha, Hunan Province, China.Methods Polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were used for genotyping of FII rs1799963 and FV rs6025 in 351 patients diagnosed with cerebral infarction (patient group) and 417 healthy individuals undergoing physical examination (control group). All participants were Han Chinese selected from the Department of Neurology, Third Xiangya Hospital of Central South University, from June 2011 to July 2012. The distribution of genotypes and allele frequency were analyzed by the χ² test. The relationship between selected gene polymorphisms and cerebral infarction was examined by logistic regression analysis.Results The genotypes of FII rs1799963 were G/G in the patient group and G/G plus G/A in the control group. The genotype of FV rs6025 was G/G in all participants. The distribution of genotypes and allele frequency at FII rs1799963 showed no significant difference between the patient and control groups (P>0.05). The gene polymorphisms of FII rs1799963 had no association with cerebral infarction (P>0.05). Conclusions There may exist no associati.Conclusions on between the gene polymorphisms of blood coagulation factors (FII rs1799963 and FV rs6025) and cerebral infarction in the Han Chinese population from Changsha.