Objective To study the therapeutic effect of combined use of clopidogrel and aspirin in the treatment of acute cerebral infarction by measuring the expression of platelet membrane glycoprotein and lysosome membrane protein 53 in patients with cerebral infarction.Methods Sixty patients with cerebral infarction were randomly divided into two groups: aspirin alone (0.15 g/d) and aspirin (0.10 g/d)+clopidogrel (75 mg/d). Thirty healthy persons were used as control group. Flow cytometry was employed to detect CD62p and CD63 expression on circulating platelets. NIHSS scores were evaluated.Results The positive percentage of CD62p and CD63 on platelets significantly increased in patients with cerebral infarction than in controls. The positive percentages of CD62p and CD63 on platelets and NIHSS scores were significantly reduced in patients with cerebral infarction in both aspirin alone and aspirin+clopidogrel groups after 1 and 2 weeks of treatment. The group receiving a combined use of aspirin and clopidogrel showed a lower positive percentage of CD 62p and CD63 on platelets and a lower NIHSS score than the aspirin alone group 2 weeks after treatment(P<0.01).Conclusions Antiplatelet treatment is effective in patients with cerebral infarction. A combination of aspirin and clopidogre for treatment of cerebral infarction appears to be superior to aspirin alone. CD62p and CD63 expression on the surface of plateles can be used for the evaluation of the efficacy of antiblatelet agents.