Objective To study the tropism of rat bone marrow mesenchymal stem cells(BMSCs) differentiated into neural cells towards C6 glioma cells in vitro.Methods Firstly BMSCs were induced to differentiated into neural cells through adding the appropriate inducing factors into condition medium (such as 2% B27, 20ng/ml bFGF, 20ng/mlEGF and so on), and then the neurally differentiated BMSCs and C6 glioma cells were co-cultured with transwell system. The number of migrated cells was counted by statistical software.Results Rat BMSCs successfully differentiated into neural cells (Nestin (24.3±5.2)%,NSE (33.6±3.8)% and NeuN (41.9±4.7)%), and transwell co-culture test manifested the number of migrated cells in experimental group was more significant than the which in control group.Conclusions Neurally differentiated rat BMSCs have obviously tropism towards C6 glioma cells in vitro.