The primers selected in this study are complementary to the common conserved sequences of gene 9 (or 8) a- mong A group four rotavirus serotypes.The method of polymerase chain reaction (PCR) for detection of group A rotavirus nucleic acid in faecal samples.About 342 nu- clcotides were amplified with them.We have tested 89 faecal samples of 19 cases rotavirus asymptomatic infec- tion of neonates by using reverse transcrip-tase-polyme- rase chain reaction (RT-PCR) and for comparision with polyacrylamide gel electrophoresis (PAGE).Of 89 fae- cal samples from the 18 neonates,54(60.1%)con- tained rotavirus RNA detected by RT-PCR,whereas only 35 (38.1%) were detected for rotavirus RNA by PAGE.the result probably close to the actual situation of the neonates shedding rotavirus.